Trial Parameters
Brief Summary
There is reason to believe the current dietary reference intakes for dietary protein for children are too low. Furthermore, children with high levels of physical activity and fitness may require a higher requirement. To better understand the protein requirements in minimally and highly physically fit and active children 8-10 y old, investigators will use the indicator amino acid oxidation technique.
Eligibility Criteria
Inclusion Criteria: * Inclusion Criteria * Boys or girls * Ages 8-10 years * All races * All ethnicities * Children who were determined to have normal weight (BMI \<85th percentile) already determined under Mitochondria (MI) Energy (IRB Protocol: 260376) * Participants willing to stop taking nutritional supplements (e.g., multivitamins, vitamin D, fish oil, probiotics, prebiotics, immune boosters, and others) for at least 2 weeks prior to each study testing visit * Children without an infection requiring antibiotics willing to be rescheduled after at least 2 months of finalizing antibiotic treatment. * Children without viral infections such as diarrhea, cold, or flu willing to be rescheduled after at least 2 weeks of resolution of symptoms. * Children determined sedentary or active, based on both peak oxygen uptake (VO2) and accelerometer data, as described below: Children who completed a peak fitness test during MI Energy (IRB Protocol: 260376), and for whom peak VO2 data are as follo