Molecular-genetic Characterization in Patients Undergoing CAR-T Cell Infusion
This study examines the genetic and molecular characteristics of patients receiving CAR-T cell therapy, a treatment that uses specially engineered T cells from the immune system to fight blood cancers. Researchers will analyze blood samples and genetic information to better understand how these modified cells work and respond in different patients.
Key Objective: This research aims to improve understanding of how CAR-T cell therapy works at the genetic level, which could help doctors better predict treatment outcomes and customize therapy for individual patients.
Who to Consider: Patients with blood-related cancers (such as lymphoma or leukemia) who are undergoing or planning to undergo CAR-T cell therapy should consider enrolling to contribute to medical knowledge about this treatment.
Trial Parameters
Brief Summary
In recent years, the application of increasingly advanced methods of ex-vivo cell culture and cell engineering has made it possible to develop new cellular therapeutic platforms including the "CAR (Chimeric Antigen Receptor) - T cell therapy". CAR-T cell therapy is a therapy that uses T lymphocytes engineered to express a chimeric receptor directed against a specific antigen, theoretically applicable to the treatment of all neoplasms but currently more widely used in the treatment of haematological malignancies. One of the most innovative aspects introduced with CAR-T cell therapy is that of living-drug, cells that act as a drug as well as a means to build specific immunity against the neoplasm. The advantages of this therapy are therefore represented by the possibility of refueling the patient's immunity, deficient in the control of the neoplastic disease, with lymphocytes capable of expressing an antineoplastic activity with mechanisms not subject to restriction of HLA-mediated antigen recognition. However, the use of CAR-T therapies is not free from potentially serious and sometimes lethal adverse events; in the toxicity profile the following are recognizable as peculiar: * cytokine release syndrome (CRS) * B-cell aplasia (hypogammaglobulinemia) * neurological adverse reactions * haematological toxicity * infections. Therefore, considering that on the one hand adverse events are not negligible and on the other hand that a percentage \> 50% of patients lose the response obtained, it is necessary to improve the therapeutic profile of CAR-T cell therapy by increasing its efficacy and reducing its toxicity . Both of these strategies are linked to the understanding of the resistance mechanisms of neoplastic cells, as well as to the biology of CAR-T cells and of all the cellular (microenvironment) and non-cellular systems with which they interact.
Eligibility Criteria
Inclusion Criteria: 1. Patients aged ≥ 18 years. 2. Patients with haematological pathology hospitalized for CAR-T cell infusion therapeutic program (with Marketing Authorization) at the Departmental Program of Advanced Cellular Therapies, of the IRCCS AOU of Bologna 3. Patients with express consent to participate in this study, acquired by signing the informed consent. Exclusion Criteria: \-