Collection of Lung Fluid and Tissue Samples for Research
Trial Parameters
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Brief Summary
This study will collect fluid and tissue specimens from the lungs and nose of healthy people and people with a history of lung infections. The specimens will be examined for differences between the two groups that may be associated with susceptibility to certain infections. Healthy normal volunteers and people with a history of lung infections between 18 and 75 years of age who are followed at NIH may be eligible for this study. Participants undergo the following procedures: Medical history and physical examination. Blood and urine tests. Electrocardiogram (ECG) and chest x-ray. Treadmill exercise stress test (for people over 45 years old with a history of chest pain or ECG abnormalities). Bronchoscopy: The subject s nose and throat are numbed with lidocaine and a sedative is given for comfort. A thin flexible tube called a bronchoscope is advanced through the nose or mouth into the lung airways to examine the airways carefully. Fluid collection during the bronchoscopy using one of the following methods: * Bronchoalveolar lavage: Salt water is injected through the bronchoscope into the lung and immediately suctioned out, washing off cells lining the airways. * Bronchial brushings: A brush-tipped wire enclosed in a sheath is passed through the bronchoscope and a small area of the airway tissue is gently brushed. The brush is withdrawn with some tissue adhering to it. * Endobronchial biopsies: Small pinchers on a wire are passed through the bronchoscope and about 1 to 2 millimeters of tissue is removed. * Nasal scrape: A small device is used to scrape along the inside of the nose to collect some cells. * Sputum induction * Exhaled breath condensate to obtain specimens for in vitro investigations and comparisons of both the cellular and acellular components. * Nasal nitric oxide production * Nasal potential difference * Exhaled aerosol mask sample collection * Cough aerosol collection * Exhaled particle collection * Lung Clearance Index (LCI)
Eligibility Criteria
* HEALTHY VOLUNTEER INCLUSION CRITERIA FOR BRONCHOSCOPY AND BRONCHOSCOPIC PROCEDURES: 1. 18 to 75 years of age inclusive 2. Enrolled without regard to gender, race, or ethnicity 3. NIH staff are eligible 4. Able to provide proof of identity to the satisfaction of the study clinician completing the enrollment process 5. Able and willing to complete the informed consent process 6. Able and willing to arrange to have another person drive them home after the procedure 7. Able and willing not to eat or drink anything for 6 hours prior and 2 hours after the procedure 8. Willing to donate blood and respiratory tract samples for storage to be used for future research 9. In good general health without clinically significant medical history 10. Physical examination without clinically significant findings 11. Screening laboratory tests without clinically significant abnormalities: * Complete blood count with differential * Serum chemistries including creatinine, blood urea nitrogen, glucose, live