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Recruiting Phase 1 NCT07169487

NCT07169487 Adjunctive Methylene Blue for Immunotherapy-related CRS and ICANS: Phase I Study

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Clinical Trial Summary
NCT ID NCT07169487
Status Recruiting
Phase Phase 1
Sponsor Institute of Hematology & Blood Diseases Hospital, China
Condition Cytokine Release Syndrome
Study Type INTERVENTIONAL
Enrollment 18 participants
Start Date 2025-06-28
Primary Completion 2028-06-28

Eligibility & Interventions

Sex All sexes
Min Age 18 Years
Max Age N/A
Study Type INTERVENTIONAL
Interventions
Methylene Blue

Eligibility Fast-Check

Enter your details for a quick preliminary check. This does not replace medical advice.

What to Expect as a Participant

You will actively receive the study intervention — which may be a drug, biologic, device, or procedure.

Phase 1 is the earliest stage of human testing — safety and dosage are the primary focus. Visits are frequent and medical supervision is intensive. You will be among the first people to receive this treatment.

This trial targets 18 participants in total. It began in 2025-06-28 with a primary completion date of 2028-06-28.

⚠ This information is for research awareness only. Always consult your physician before joining any clinical trial. Participation is voluntary and you may withdraw at any time.

Brief Summary

This Phase I, prospective, single-arm clinical study aims to evaluate the efficacy and safety of adjunctive methylene blue (MB) in patients experiencing cytokine release syndrome (CRS) or immune effector cell-associated neurotoxicity syndrome (ICANS) following CAR-T cell therapy or bispecific antibody treatment. Preclinical studies demonstrated that MB alleviates CRS/ICANS-related symptoms, preserves the antitumor function of T cells, and modulates neuroinflammation without compromising immune efficacy. The study will employ a 3+3 dose-escalation design with three MB dosing cohorts, with treatment administered intravenously for 3-5 consecutive days. Vital signs, laboratory markers, and neurological status will be closely monitored, and concomitant standard supportive therapies will be permitted.

Eligibility Criteria

Inclusion Criteria: 1. Diagnosed with hematologic malignancies based on cytomorphology and immunophenotyping; age ≥18 years. 2. Received immunotherapy (e.g., CAR-T cells, bispecific antibodies) and developed CRS or ICANS of ASTCT Grade ≥1. 3. Estimated life expectancy ≥3 months. 4. Male and female participants of childbearing potential agree to use effective contraception. 5. Left ventricular ejection fraction (LVEF) \>45% by echocardiography. 6. Ability to understand and sign informed consent and willingness to comply with study requirements. Exclusion Criteria: 1. Glucose-6-phosphate dehydrogenase (G6PD) deficiency. 2. Known allergy to methylene blue. 3. Pregnant or breastfeeding women. 4. Known HIV seropositivity. HIV testing may be required according to local laws or regulations. 5. History of clinically significant ventricular arrhythmia, unexplained syncope (not vasovagal), sinoatrial block, or higher-degree atrioventricular (AV) block with chronic bradycardia (unless a permanent pacemaker is implanted). 6. Psychiatric disorders that may interfere with completion of treatment or informed consent. 7. Any other condition deemed unsuitable for participation by the investigator.

Contact & Investigator

Central Contact

Jianxiang Wang

✉ wangjx@ihcams.ac

📞 +862223909120

Principal Investigator

Jianxiang Wang

PRINCIPAL INVESTIGATOR

Institute of Hematology & Blood Diseases Hospital, China

Frequently Asked Questions

Who can join the NCT07169487 clinical trial?

This trial is open to participants of all sexes, aged 18 Years or older, studying Cytokine Release Syndrome. Full inclusion and exclusion criteria are listed in the Eligibility Criteria section. Always confirm your eligibility with the research team before applying.

What phase is the NCT07169487 trial and what does that mean for participants?

Phase 1 trials are the first stage of human testing. The primary goal is to assess safety and determine appropriate dosage levels. Participants are closely monitored. These trials typically involve a small number of volunteers.

Is NCT07169487 currently recruiting?

Yes, NCT07169487 is actively recruiting participants. Contact the research team at wangjx@ihcams.ac for enrollment information.

Where is the NCT07169487 trial being conducted?

This trial is being conducted at Tianjin, China.

Who is sponsoring the NCT07169487 clinical trial?

NCT07169487 is sponsored by Institute of Hematology & Blood Diseases Hospital, China. The principal investigator is Jianxiang Wang at Institute of Hematology & Blood Diseases Hospital, China. The trial plans to enroll 18 participants.

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ClinicalMetric — Independent clinical trial intelligence platform. Not affiliated with NIH, ClinicalTrials.gov, the U.S. FDA, or any pharmaceutical company, hospital, or clinical research organization. Trial data is sourced from ClinicalTrials.gov for informational purposes only and does not constitute medical advice. Do not make any treatment, enrollment, or health decisions based solely on information found here — always consult a qualified healthcare professional. Full Disclaimer  ·  Last Reviewed: July 2026  ·  Data Methodology