Trial Parameters
Brief Summary
In this project the investigators will test if it is possible to measure changes in intestinal gas production after supplementation of a complex fiber mixture over a 36 hour period in both lean normoglycemic individuals and individuals with insulin resistance and/or prediabetes with overweight when compared with a placebo Changes in intestinal gas production will also be related to energy expenditure, substrate metabolism, microbial composition and related metabolites in feces, blood and urine.
Eligibility Criteria
Inclusion Criteria: * Lean normoglycemic individuals: * Lean (BMI ≥ 18.5 kg/m2 and ≤ 24.9 kg/m2 ); * Normal fasting glucose (plasma glucose \< 5.6 mmol/L) and a HOMA-IR\< 2.2 Individuals with overweight/obesity and prediabetes/insulin resistance: * Overweight/obesity (BMI ≥ 28 kg/m2 and ≤ 40 kg/m2 ); * insulin resistance (HOMA-IR\>2.2) and/or impaired fasting glucose (IFG: plasma glucose ≥ 5.6 mmol/l) For both subject groups: * Aged 30 - 75 years; * Normal blood pressure (systolic blood pressure 100-150mmHg, diastolic blood pressure 60-100 mmHg); * Weight stable for at least 3 months (± 2 kg) Exclusion Criteria: * T2DM (defined as fasting plasma glucose ≥ 7.0 mmol/L) * Gastroenterological diseases * Abdominal surgery affecting the GI tract; * Cardiovascular diseases, cancer, liver or kidney malfunction, disease with a life expectancy shorter than five years; * Abuse of products; * Alcohol (\> 15 standard units per week) * Drugs * Excessive nicotine use defined as \>20 cigarettes per da